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The allosteric transcription factor TetR acts as a sensor and actuator in this quantum dot enabled fluorescent bead assay for the small molecule anhydrotetracycline. This rapid, antibody‐free biosensor produces a visible change in fluorescent color, which can be detected by eye or with an inexpensive digital camera, based on dose‐dependent analyte‐responsive protein‐DNA binding. The adaptable sensor uses easily accessible biomolecular components. Abstract. Recently, allosteric transcription factors (TFs) were identified as a novel class of biorecognition elements for in vitro sensing, whereby an indicator of the differential binding affinity between a TF and its cognate DNA exhibits dose‐dependent responsivity to an analyte. Described is a m.
Huffington Post 09/18/2020 07:04
, a bestselling author of fantasy books, including the epic series The Sword of Truth , died on Thursday. Cause of death was not released. He was 72. Goodkind’s representatives confirmed his death on Facebook, calling his passing an “unimaginable loss” for the publishing industry:. He also fueled backlash after because it featured an image of a female character. But fans regularly treated Goodkind’s work as canon within the fantasy sphere. Readers and fellow authors flocked to and social media to remember the author:. I just heard that Terry Goodkind has died. I never got to meet him, but he and I, Robert Jordan and Terry Brooks always seemed to be following around the country on our tours. My condolences to those who loved him, and to those

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