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Zymomonas mobilis is a promising biofuel producer due to its high alcohol tolerance and streamlined metabolism that efficiently converts sugar to ethanol. Z. mobilis genes are poorly characterized relative to model bacteria, hampering our ability to rationally engineer the genome with pathways capable of converting sugars from plant hydrolysates into valuable biofuels and bioproducts. Many of the unique properties that make Z. mobilis an attractive biofuel producer are controlled by essential genes; however, these genes cannot be manipulated using traditional genetic approaches (e.g., deletion or transposon insertion) because they are required for viability. CRISPR interference (CRISPRi) is a programmable gene knockdown system that can prec.
The outer membrane c -type cytochromes ( c -Cyts) OmcA and MtrC in Shewanella are key terminal reductases that bind and transfer electrons directly to iron (hydr)oxides. Although the amount of OmcA and MtrC at the cell surface and their molecular structures are largely comparable, MtrC is known to play a more important role in dissimilatory iron reduction. To explore the roles of these outer membrane c -Cyts in the interaction of S. oneidensis MR-1 with iron oxides, the attachment processes of S. oneidensis MR-1 wild type and c -type cytochrome-deficient mutants ( omcA, mtrC and omcA - mtrC ) to goethite are compared via quartz crystal microbalance with dissipation monitoring (QCM-D). Strains with OmcA exhibit a rapid initial attachment. Th.
Nonribosomal peptides (NRPs) are a class of secondary metabolites usually produced by microorganisms. They are of paramount importance in different applications including biocontrol and pharmacy. Brevibacillus spp. is a rich source of NRPs yet has received little attention. In this study, we characterize four novel bogorol variants (bogorol I-L, cationic linear lipopeptides) and four succilins (succilin I-L, containing a succinyl group that is attached to the Orn 3 /Lys 3 in bogorol I-L) from the biocontrol strain B. laterosporus MG64. Further investigation revealed that the bogorol family of peptides employ an adenylation pathway for lipoinitiation, which is different from the usual pattern that is based on an external ligase and coenzyme
Knowledge of the isoelectric point (pI) of viruses is beneficial for predicting virus behavior in environmental transport and physical/chemical treatment applications. However, the empirically measured pIs of many viruses have thus far defied simple explanation, let alone prediction, based on the ionizable amino acid composition of the virus capsid. Here, we suggest an approach for predicting the pI of nonenveloped viruses by excluding capsid regions that stabilize the virus polynucleotide via electrostatic interactions. This method was applied first to viruses with known polynucleotide-binding regions (PBRs) and/or 3D structures. Then, PBRs were predicted in a group of 32 unique viral capsid proteome sequences via conserved structures and
4Gamer.net (Japan) 09/25/2020 21:17
THE KING OF FIGHTERS for GIRLS THE KING OF FIGHTERS for GIRLS 配信元 ビクターエンタテインメント・ゲームズ 配信日 2020/09/25 THE KING OF FIGHTERS for GIRLS 1周年を記念した1st配信ライブが決定!! Android/iOS用格闘×恋愛ADV『THE KING OF FIGHTERS for GIRLS』(略称:KOFG)が正式サービス開始1…
Orissa POST (India) 09/25/2020 21:15
Researchers have developed a computational model which is effective in detecting and identifying genetic mutations in breast tumours. The study included results from over 3,200 patients with breast cancer. The researchers used RNA sequencing, a sensitive, precise tool which has very gradually started to be applied clinically, although not yet for breast cancer. The study, […]
Lenta.ru (Russia) 09/25/2020 21:13
Одиннадцатилетняя София Феськова представит Россию на международном конкурсе детской песни «Евровидение-2020». Конкурсантка из Санкт-Петербурга одержала победу в финале национального отборочного тура и получила право выступить на международном уровне. Победителя определили профессиональное жюри и фанаты.
Expreso Chiapas (Mexico) 09/25/2020 21:10
Londres – ¿Le gustaría tener una parte de la historia del rock? Sotheby’s está organizando una subasta online este mes de recuerdos de los Beatles para conmemorar el 50 aniversario de la ruptura de la banda británica. La venta ofrece artículos que abarcan toda la carrera de la banda, incluida una copia firmada del primer sencillo de los Fab Four de 1962, “Love Me Do”, valuada en entre 19 mil-25 mil 400 dólares. Otros objetos a la venta incluyen un par de los inconfundibles lentes de John Lennon, valuados en 30 mil a 40 mil libras, y su hoja de detención escolar, con un precio de venta estimado en 5 mil libras, que detalla un registro de mala conducta cuando era niño. Un reloj Cartier de 1966, estimado en hasta 25 mil libras, también será su.
Expreso Chiapas (Mexico) 09/25/2020 21:10
Ciudad de México – Una piedra de forma triangular marca la tumba simbólica de Mordejái Anilevich, judío socialista que junto con otros jóvenes organizó y participó en la resistencia contra las fuerzas nazis en el gueto de Varsovia. Hoy del gueto no hay nada; sin embargo, esa es una de las historias que no hay que olvidar, asegura el escritor Paco Ignacio Taibo II, quien recuperó ese fragmento de la historia en su libro Sabemos cómo vamos a morir, publicado por la editorial Planeta. El ejemplar lleva como subtítulo Una resistencia imposible: la historia del levantamiento del gueto de Varsovia; la intención de escribirlo se remonta a unos ocho o 10 años atrás, cuando Taibo II leyó una carta de Mordejái, donde escribió que no había duda de que.

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