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Pre-treatment of waste activated sludge (WAS) is an effective way to destabilize sludge floc structure and release organic matter for improving sludge digestion efficiency. Nonetheless, impacts of WAS pre-treatment on digestion sludge microbiomes, as well as mechanistic insights into how sludge pre-treatment improve digestion performance, remain elusive. In this study, genome-centric metagenomic approach was employed to investigate the digestion sludge microbiome in four sludge digesters with different feeding sludge: APAD, WAS pre-treated with 0.25 mol/L alkaline/acid; HS-APAD, WAS pre-treated with 0.8 mol/L alkaline/acid; Thermal-AD, thermal pre-treated WAS; Control-AD, fresh WAS. We retrieved 254 metagenomic-assembled genomes (MAGs) to i.
Methanotrophic microorganisms are characterized by their ability to oxidize methane. Globally they have a significant impact on methane emissions by attenuating net methane fluxes to the atmosphere in natural and engineered systems, though the populations are dynamic in their activity level in soils and waters. Methanotrophs oxidize methane using methane monooxygenase (MMO) enzymes, and selected subunit genes of the most common MMOs, specifically pmoA and mmoX , are used as biomarkers for presence and abundance of populations of bacterial methanotrophs. Relative expression of these biomarker genes is dependent on copper-to-biomass ratios. Empirically derived quantitative relationships between methane oxidation biomarker transcript amounts a.
Acetogenic bacteria are a diverse group of anaerobes which use reductive acetyl-CoA (Wood-Ljungdahl) pathway for CO 2 fixation and energy conservation. The conversion of 2 moles CO 2 into acetyl-CoA via Wood-Ljungdahl pathway being terminal electron accepting pathway is the most prominent metabolic feature for these microorganisms. However, here we describe that the fecal acetogen Clostridium bovifaecis strain BXX displayed poor metabolic capabilities of autotrophic acetogenesis, and acetogenic utilization of glucose occurred only with the supplementation of formate. Genome analysis of Clostridium bovifaecis revealed that it contains almost complete genes of Wood-Ljungdahl pathway but lacks gene encoding formate dehydrogenase, which catalyz.
Extracellular DNA (eDNA) is a biofilm component that contributes to the formation and structural stability of biofilms. Streptococcus mutans , a major cariogenic bacterium, induces eDNA-dependent biofilm formation under specific conditions. Since cell death can result in the release and accumulation of DNA, the dead cells in biofilms are a source of eDNA. However, it remains unknown how eDNA is released from dead cells and is localized within S. mutans biofilms. We focused on cell death induced by the extracellular signaling peptide, competence-stimulating peptide (CSP). We demonstrate that nucleic acid release into the extracellular environment occurs in a subpopulation of dead cells. eDNA production induced by CSP was highly dependent on
In recent years various studies have demonstrated that the gut microbiota influences host metabolism. However, these studies were primarily focused on a single or a limited range of host species, thus preventing a full exploration of possible taxonomic and functional adaptations by gut microbiota members as a result of host-microbe co-evolution events. In the current study, the microbial taxonomic profiles of 250 fecal samples, corresponding to 77 host species that cover the mammalian branch of the tree of life, were reconstructed by 16S rRNA gene-based sequence analysis. Moreover, shotgun metagenomics was employed to investigate the metabolic potential of the fecal microbiomes of 24 mammals and subsequent statistical analyses were performe.
Tribune India 09/18/2020 22:24
New Delhi. Kia Motors on Friday launched its compact SUV Sonet in India, priced between Rs 6.71 lakh and Rs 11.99 lakh. It will compete with the likes of Hyundai Venue, Maruti Suzuki Vitara Brezza, Tata Motors Nexon, Honda WR-V and Ford Ecosport, among others. PTI. Mumbai. India Ratings cuts banking sector outlook to negative. Domestic rating agency India Ratings and Research on Friday revised its outlook on the country's banking sector to negative for the second half of this fiscal from stable due to the likely increase in stressed assets, credit costs and weak earnings. PTI.
Tribune India 09/18/2020 22:22
New Delhi, September 18. Apple on Friday said it will launch its online store in India next week, offering its full range of products to customers across the country for the first time, a move that will help the tech giant cash in on the festive demand. The new online store, which will be launched on September 23, will provide customers with the same premium experience found in Apple Store locations around the world, delivered by online team members who are ready to offer their expertise, Apple said. In February, Apple CEO Tim Cook had said the company will launch its online store in India later this year, while its first physical retail store will open in the country in 2021. "We know our users are relying on technology to stay connected, e.
Tribune India 09/18/2020 22:22
Vijay C Roy. Tribune News Service. Chandigarh, September 18. The pandemic-induced lockdown, followed by trade disruption, especially fall in imports, has led to shortage of containers in Ludhiana, affecting exports from Punjab, Himachal Pradesh and Jamp;K. Due to the trade disruption, exporters have to wait for 5 to 10 days for containers as compared to 0-1 day during the pre-Covid times. 10-day waiting. Due to the trade disruption, exporters have to wait for 5 to 10 days for containers as compared to waiting period of 0-1 day during the pre-Covid times. The lockdown led to a sharp drop in imports.
Tribune India 09/18/2020 22:22
New Delhi, September 18. Telecom regulator TRAI on Friday issued a fresh set of rules for publication and advertisement of tariff plans by telcos, in a bid to boost transparency in mobile offers and to help subscribers make informed decisions. Directive to telcos. TRAI has now directed telcos to publish, service area-wise — within 15 days — each tariff plan for postpaid and prepaid subscribers. The consumer-oriented move assumes significance as telecom subscribers often grapple with issues such as lack of information, unclear or hard to find plan details — a situation that affects subscribers' ability to make proper choices. Tightening the rules for telcos, Telecom Regulatory Authority of India (TRAI) on Friday issued directions on tariff publ.
The Vox 09/18/2020 22:20
Supreme Court Justice Ruth Bader Ginsburg at Georgetown University in 2019. | Jacquelyn Martin/AP Photo. On Tucker Carlson Tonight, conservative Judge Amy Coney Barrett was floated as a replacement for Ginsburg. Less than one hour after the was announced, speculation began on Fox News about her replacement. On Tucker Carlson Tonight Friday, Ned Ryun — the CEO of American Majority, an advocacy group that trains conservatives who aspire to political office — said he would advise President Donald Trump to nominate Amy Coney Barrett, who he said, “I’m a big fan of; in fact, I prefer her over Kavanaugh ... I think she’d be a solid choice, she’s still in her 40s. She’s on the Seventh Circuit right now, has proven her conservative credentials.”Fox.
Orissa POST (India) 09/18/2020 22:19
Researchers have found that Covid-19 may be diagnosed on the same emergency scans intended to diagnose stroke. The findings published in the American Journal of Neuroradiology, have important implications in the management of patients presenting with suspected stroke through early identification of Covid-19. In the study, the research team from King’s College London in the […]
Fiji Times (Fiji) 09/18/2020 22:19
The Premier League has urged the British government to stay committed to its plans for a controlled return of fans into stadiums from the start of October. The government last week restricted pilot events to 1,000 spectators after a spike in COVID-19 cases, and said it would review the date for when fans could enter […]


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