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PubMed News (NIH) 02/21/2020 23:00
Contributors : Sahar Houshdaran ; Linda C Giudice ; Ashwini B Oke ; Jennifer C Fung ; Kim C Vo ; Camran Nezhat Series Type : Methylation profiling by genome tiling array Organism : Homo sapiens. Genome-wide DNA methylation profiling of hormone treated normal and endometriosis stromal fibroblast cells (eSF).
PubMed News (NIH) 02/21/2020 23:00
Contributors : Qiang Zhao ; Jia Xue ; Wubin Qian ; Tiezhu Liu ; Bin Fan ; Jie Cai ; Yongpeng Ji ; Jia Liu ; Yong Yang ; Qi-Xiang Li ; Sheng Guo ; Ning Zhang Series Type : Expression profiling by high throughput sequencing Organism : Homo sapiens. Large-scale initiatives like The Cancer Genome Atlas (TCGA) performed omics studies on hundreds of kidney cancer patients, but predominantly on Caucasians.
PubMed News (NIH) 02/20/2020 23:00
Contributor : Allen R Buskirk Series Type : Expression profiling by high throughput sequencing ; Other Organism : Escherichia coli str. K-12 substr. MG1655. Shine-Dalgarno (SD) motifs are thought to play an important role in translational initiation in bacteria. Paradoxically, ribosome profiling studies in E. coli show no correlation between the strength of the SD in an mRNA and how efficiently it is translated. Performing profiling on ribosomes with altered anti-Shine-Dalgarno sequences, we reveal a genome-wide correlation between SD strength and ribosome occupancy that was previously masked by other contributing factors. Using the antibiotic retapamulin to trap initiation complexes at start codons, we find that the mutant ribosomes select
PubMed News (NIH) 02/17/2020 23:00
Contributor : Serge McGraw Series Type : Methylation profiling by high throughput sequencing Organism : Mus musculus. In early embryos, DNA methylation is remodelled to initiate the developmental program. For mostly unknown reasons, methylation marks are acquired unequally between embryonic and placental cells. To better understand this, we generated high-resolution maps of DNA methylation in mouse mid-gestation (E10.5) embryo and placenta. We uncovered specific subtypes of differentially methylated regions (DMRs) that contribute directly to the developmental asymmetry existing between mid-gestation embryo and placenta. We show that the asymmetry between embryonic and placental DNA methylation patterns occurs rapidly during the acquisition o.

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