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PubMed News (NIH) 10/21/2019
Contributors : Yiren Xiao ; Subarna Sinha ; Erinn B Rankin Series Type : Expression profiling by high throughput sequencing Organism : Homo sapiens. Purpose: FTO is an N6-methyladenosine (m6A) RNA demethylase. The goals of this study are to to identify functional mRNA targets of FTO m6A demethylase activity in VHL deficient ccRCC cells. We performed transcriptome wide m6A sequencing and RNA sequencing analysis of two independent FTO deficient and wild type ccRCC cell lines. Methods: We performed transcriptome wide m6A sequencing and RNA sequencing analysis of two independent FTO deficient (shFTO#2 and shFTO#5) and wild type ccRCC cell lines. Results: Analysis using exomePeak identified a total of 20,168 m6A peaks that are different between t.
PubMed News (NIH) 10/21/2019
Contributors : Li Wang ; Peng Yu ; Zheng Li ; Zongna Ren Series Type : Expression profiling by high throughput sequencing Organism : Homo sapiens. We studied the cell compositon of two types of human heart disease (coronary atherosclerotic heart disease and dilated cardiomyopathy) by single-cell sequencing.
PubMed News (NIH) 10/21/2019
Contributors : Li Wang ; Peng Yu ; Zheng Li ; Zongna Ren Series Type : Expression profiling by high throughput sequencing Organism : Homo sapiens. We studied the cell compositon of normal human heart by single-cell sequencing. Distint subgroups of cardiac muscle, fibroblast cell and endothelial cell were detected. We drawed a cell-cell interaction network using specific expressed ligands and receptors of cells. And we also observed the change of interaction and cell transformation with age.
PubMed News (NIH) 10/21/2019
Contributors : Alex Chialastri ; Siddharth S Dey ; Joanna J Gell ; Wanlu Liu ; Enrique Sosa ; Grace Hancock ; Yu Tao ; Sissy E Wamaitha ; Grace Bower ; Amander T Clark Series Type : Methylation profiling by high throughput sequencing Organism : Homo sapiens. In this study, we evaluated the 5mC content of single cell human primordial germ cell-like cells (hPGCLCs) generated from a male human embryonic stem cell line (UCLA2, 46XY).
PubMed News (NIH) 10/21/2019
Contributors : Inés Reverón ; Laura Plaza ; Blanca de las Rivas ; Rosario Muñoz ; Félix López de Felipe Series Type : Expression profiling by array Organism : Lactobacillus plantarum. Whole genome transcriptional profiling was used to characterize the response of Lactobacillus plantarum WCFS1 human isolate during challenge with oleuropein.
PubMed News (NIH) 10/21/2019
Contributors : Ilham H Said-Salman ; Ftaima A Jebaii Series Type : Expression profiling by high throughput sequencing Organism : Escherichia coli. Purpose: In this study, Escherichia coli DH5alpha whole transcriptome sequencing was performed in order to compare the different gene expression profiles between control and exposed to Wi-Fi radiofrequency radiations.
PubMed News (NIH) 10/21/2019
Contributors : Eleni Tzima ; Iliana Serifi ; Thomais Papamarcaki Series Type : Expression profiling by array Organism : Danio rerio. To analyse the gene expression profile of zebrafish in response to nutritional supplementation with mastic essential oil, mRNA was isolated at 42dpf and subjected to DNA microarray analysis.
PubMed News (NIH) 10/21/2019
Contributors : Charles P Fulco ; Joseph Nasser ; Thouis Jones ; Glen Munson ; Drew T Bergman ; Vidya Subramanian ; Sharon R Grossman ; Rockwell Anyoha ; Tejal Patwardhan ; Tung H Nguyen ; Michael Kane ; Benjamin Doughty ; Elizabeth M Perez ; Neva C Durand ; Elena K Stamenova ; Erez L Aiden ; Eric S Lander ; Jesse M Engreitz Series Type : Expression profiling by high throughput sequencing Organism : Homo sapiens. Mammalian genomes harbor millions of noncoding elements called enhancers that quantitatively regulate gene expression, but the mechanisms that determine which enhancers regulate which genes are not well understood.
PubMed News (NIH) 10/21/2019
Contributors : Anne-Claire Martines ; Albert Gerding ; Sarah Stolle ; Marcel A Vieira-Lara ; Justina C Wolters ; Angelika Jurdzinski ; Laura Bongiovanni ; Alain de Bruin ; Pieter van der Vlies ; Gerben van der Vries ; Vencent W Bloks ; Terry G Derks ; Dirk-Jan Reingoud ; Barbara M Bakker Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. During fasting, mitochondrial fatty-acid β-oxidation (mFAO) is essential for the generation of glucose by the liver.
PubMed News (NIH) 10/21/2019
Contributors : Vasti T Juárez-González ; Brenda A López-Ruiz ; Patricia Baldrich ; Blake C Meyers ; Tzvetanka D Dinkova ; Brenda A López-Ruiz Series Type : Expression profiling by high throughput sequencing Organism : Zea mays. We report the role of sRNAs populations during the induction of callus tissues from VS-535 maize embryos displaying contrasting in vitro embryogenic potential; characterized through Next-generation sequencing (NGS).
PubMed News (NIH) 10/21/2019
Contributors : François Fauteux ; Yunli Wang ; Hélène Rocheleau ; Ziying Liu ; Youlian Pan ; George Fedak ; Curt McCartney ; Thérèse Ouellet Series Type : Expression profiling by high throughput sequencing Organism : Triticum aestivum. In this study, early infection by F. graminearum was characterized in a doubled-haploid population derived from the cross of the moderately FHB resistant wheat genotypes Wuhan 1 and Nyubai.
PubMed News (NIH) 10/21/2019
Contributors : Daoquan Xiang ; Raju Datla ; Youlian Pan ; Ziying Liu Series Type : Expression profiling by high throughput sequencing Organism : Aegilops speltoides ; Aegilops tauschii ; Triticum aestivum ; Triticum monococcum ; Triticum turgidum. we present an atlas of global gene expression as well as the evolutionary divergence covering embryo, endosperm and seed coat development in wheats and their diploid ancestors, providing insights into the evolution of gene expression in embryogenesis and grain development of wheat species.

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