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RNA-Seq Blog 06/14/2019 02:00
The blood and immune system are characterised by utmost diversity in its cellular components. This heterogeneity can solely be resolved with the application of single-cell technologies that enable precise examination of cell-to-cell variation. Single-cell transcriptomics is continuously pushing forward our understanding of processes driving haematopoiesis and immune responses in physiological settings as well as in ...
RNA-Seq Blog 06/14/2019 01:00
Aphids and the plant viruses they transmit cause billions of dollars in crop damage around the world every year. Researchers in Michelle Heck’s lab at the USDA Agricultural Research Service and Boyce Thompson Institute are examining the relationship at the molecular level, which could lead to new methods for controlling the pests. Heck’s group used ...
RNA-Seq Blog 06/13/2019 02:00
Sometimes, analyzing our DNA sequences isn’t quite enough. RNA-seq is a next-generation sequencing (NGS)-based technique that looks at the RNA transcripts produced from our DNA, allowing us to look into how our genes are expressed. RNA-seq has shown its worth across many fields, and neuroscience is no exception. In this infographic, we’ll investigate RNA-seq, how ...
RNA-Seq Blog 06/13/2019 01:00
In a study published in Nature on June 10, researchers from Dr. YANG Hui’s Lab at the Institute of Neuroscience of the Chinese Academy of Sciences (CAS), and collaborators from the CAS-MPG Partner Institute for Computational Biology of CAS and Sichuan University demonstrated that DNA base editors generated tens of thousands of off-target RNA single nucleotide variants ...
RNA-Seq Blog 06/12/2019 02:00
RNA amplification has extensive applications on biochemistry and its related fields. Here, researchers at the Shaanxi Normal University present an isothermal strategy named rolling circle reverse transcription-mediated RNA amplification (RCRT-MRA) to amplify small RNAs with accurate length and sequence. The target RNA and complementary DNA were circularized to serve as amplicons replicated via the rolling ...
RNA-Seq Blog 06/11/2019 02:00
Next-generation DNA sequencing (NGS) is rapidly becoming an indispensable tool for genome-directed cancer diagnostics, but next-generation RNA sequencing (RNA-seq) is currently not standardly used in clinical diagnostics for expression assessment. However, multi-gene RNA diagnostic assays are used increasingly in the routine diagnosis of early stage breast cancer. Two of the most widely used tests are ...
RNA-Seq Blog 06/11/2019 01:00
Analyzing RNA sequencing data pinpoints ‘somatic clones,’ including some harboring cancer mutations, in many types of normal tissue. Cell division is not perfect. As we get older, mutations often appear in genes in normal cells. Most of these mutated cells and their progeny — called “somatic clones” — have no effect on our health, but ...
RNA-Seq Blog 06/10/2019 02:00
In small RNA (smRNA) sequencing studies, highly abundant molecules such as adapter dimer products and tissue-specific microRNAs (miRNAs) inhibit accurate quantification of lowly expressed species. Researchers from the HudsonAlpha Institute for Biotechnology previously developed a method to selectively deplete highly abundant miRNAs. However, this method does not deplete adapter dimer ligation products that, unless removed ...
RNA-Seq Blog 06/06/2019 02:00
The rapid increase in High-throughput sequencing of RNA (RNA-seq) has led to tremendous improvements in the detection and reconstruction of both expressed coding and non-coding RNA transcripts. Yet, the complete and accurate annotation of the complex transcriptional output of not only the human genome has remained elusive. One of the critical bottlenecks in this endeavor ...
RNA-Seq Blog 06/05/2019 02:00
Long INterspersed Elements-1 (LINEs/L1s) are repetitive elements that can copy and randomly insert in the genome resulting in genomic instability and mutagenesis. Understanding the expression patterns of L1 loci at the individual level will lend to the understanding of the biology of this mutagenic element. This autonomous element makes up a significant portion of the ...
RNA-Seq Blog 06/05/2019 01:00
June 17, 2019 to June 20, 2019 Registration occurs on a first-come, first-served basis. The deadline for registration is one week before the first day of the course. If you are unable to register before the deadline, please email: training@faes.org or call 301-496-7977 for space availability. NIH employees paying with the signed vendor copy of the SF-182 form, please email: registration@faes.org ...
RNA-Seq Blog 06/04/2019 02:00
Dynamic and reversible RNA modifications such as N6-methyladenosine (m6A) can play important roles in regulating messenger RNA (mRNA) splicing, export, stability and translation. Defective mRNA modification through altered expression of the methyltransferase and/or demethylases results in developmental defects and cancer progression. Identifying modified mRNAs, annotating the distribution of modification sites across the mRNA, as well ...
RNA-Seq Blog 06/04/2019 01:00
ANN ARBOR—A new way to cleanly separate out cancer cells from a blood sample enables comprehensive genetic profiling of the cancer cells, which could help doctors target tumors and monitor treatments more effectively. It is a dramatic improvement over current approaches because it also encompasses the variation among cancer cells within a single patient. “This ...
RNA-Seq Blog 06/03/2019 01:00
11-15 November 2019 – Freie Universität Berlin- Altensteinstr. 40, 14195 Berlin Course Overview RNA-Seq technology has been transformative in our ability to explore gene content and gene expression in all realms of biology, and de novo transcriptome assembly has enabled opportunities to expand transcriptome analysis to non-model organisms. This workshop provides an overview of modern ...
RNA-Seq Blog 05/31/2019 02:00
Next generation sequencing techiques revolutionized the study of RNA expression by permitting whole transcriptome analysis. However, sequencing reads generated from nested and multi-copy genes are often either misassigned or discarded, which greatly reduces both quantification accuracy and gene coverage. Here researchers at the Université de Sherbrooke present CoCo, a read assignment pipeline that takes into ...
RNA-Seq Blog 05/31/2019 01:00
There is growing evidence that alternative splicing (AS) plays an important role in cancer development. However, a comprehensive analysis of AS signatures in kidney renal clear cell carcinoma (KIRC) is lacking and urgently needed. It remains unclear whether AS acts as diagnostic biomarkers in predicting the prognosis of KIRC patients. In the work, gene expression ...

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