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PubMed News (NIH) 08/11/2020
Contributor : Sebastian Scheer Series Type : Genome binding/occupancy profiling by high throughput sequencing Organism : Mus musculus. Hhex encodes a homeobox transcriptional regulator important for embryonic development and hematopoiesis. Hhex is highly expressed in NK cells and its germline deletion results in significant defects in lymphoid development, including NK cells. To determine if Hhex is intrinsically required throughout NK cell development or for NK cell function, we generated mice that specifically lack Hhex in NK cells. NK cell numbers was dramatically reduced, while NK cell differentiation, IL-15 response and function at the cellular level remained largely normal in the absence of Hhex. Increased IL-15 availability failed to
PubMed News (NIH) 08/11/2020
Contributors : Magali De Koninck ; Elenonora Dapi ; Claudio Badia-Careaga ; Itziar Cossío ; Daniel Giménez-Llorente ; Miriam Rodríguez-Corsino ; Elena del Pilar Andrada ; Andrés Hidalgo ; Miguel Manzanares ; Francisco X Real ; Ana Losada Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. STAG2 is commonly mutated in cancer and germline mutations have been identified in cohesinopathy patients.
PubMed News (NIH) 08/11/2020
Contributors : Tomasz Chelmicki ; Aurelie Teissandier Series Type : Genome binding/occupancy profiling by high throughput sequencing Organism : Mus musculus. Using a genome-scale CRISPR knockout screening in mouse embryonic stem cells, we identify m6A RNA methylation as an important regulatory component that restricts the activity of endogenous retroviruses of the IAP (intracisternal A-particles) family.
PubMed News (NIH) 08/11/2020
Contributors : Liu Chuan ; Hu G Hua Series Type : Expression profiling by array ; Non-coding RNA profiling by array Organism : Homo sapiens. To explore the mechanisms underlying the radioresistance of hypopharyngeal carcinoma, we first established specifically radioresistant FaDu cell line (FaDu-RR cells) derived from FaDu cell lines by repeatedly exposing to different doses of ionizing radiation.
PubMed News (NIH) 08/11/2020
Contributors : Fang Zemin ; Wang Xiaojie Series Type : Expression profiling by high throughput sequencing Organism : Medicago truncatula. To investigate the gene expression levels of Medicago truncatula roots after beneficial fungi Gongronella sp. w5 inoculated.Gongronella sp. w5 promoted M. truncatula growth and caused the accumulation of sucrose in M. truncatula root tissue at 16 day-post-inoculation (dpi) without invading into the root cells. The transport of photosynthetic product sucrose to the rhizosphere by M. truncatula root cells was accelerated by upregulating the SWEET gene.
PubMed News (NIH) 08/11/2020
Contributors : James A Timmons ; Stuart M Phillips Series Type : Expression profiling by array Organism : Homo sapiens. The annotation of the Affymetrix HTA 2.0 array was updated to optimise the detection of RNA in human skeletal muscle biopsy samples by removing invalid and low signal-high-variance probes (as for CDF GPL24047).
PubMed News (NIH) 08/11/2020
Contributors : María J Garzón ; Paula Navarrete ; Sheila Lorente-Pozo ; Salvador Mena-Mollá ; Máximo Vento ; Federico V Pallardó ; José L García-Giménez Series Type : Methylation profiling by genome tiling array Organism : Homo sapiens. Neonatal sepsis is an important health-care concern worldwide occurring more frequently in premature newborns and for which diagnosis is yet a challenge, causing delays in therapy and increasing the risk of death.
PubMed News (NIH) 08/11/2020
Contributors : Stacey L Borrego ; Peter Kaiser Series Type : Expression profiling by high throughput sequencing Organism : Homo sapiens. Methionine is an essential amino acid and critical precursor to the cellular methyl donor S-adenosylmethionine. Unlike non-transformed cells, cancer cells have a unique metabolic requirement for methionine and are unable to proliferate in growth media where methionine is replaced with its metabolic precursor, homocysteine. This metabolic vulnerability is common among cancer cells regardless of tissue origin and is known as “methionine dependence”, “methionine stress sensitivity”, or Hoffman effect. Here, we characterize the response of lipids to methionine stress in the triple negative breast cancer cell li.
PubMed News (NIH) 08/11/2020
Contributor : Shu Yuan Series Type : Expression profiling by high throughput sequencing Organism : Oryza sativa. Through transcriptomic profiling, three auxin-related genes (IAA24, PIN1C, and YUC5), one GA-related gene (GA20ox2), one cell wall metabolic process gene (G4OM1), and one defense response gene (DRP-RG3) were found, which may have vital association to illuminate the function of OsDGK1.
PubMed News (NIH) 08/11/2020
Contributors : Jiang Li ; Yina Liu Series Type : Expression profiling by high throughput sequencing Organism : Homo sapiens. Exhausted T cells (TExh) is a barrier for cancer immunotherapy, evidences demonstrate that transcriptional regulation is essential for TEX induction; however the factors that manipulate exhausted phenotype are largely unexplored.
PubMed News (NIH) 08/11/2020
Contributors : Adi Bercovich-Kinori ; Ma'ayan Israeli ; Inbar Cohen-Gihon ; Ofir Israeli ; Ofer Cohen Series Type : Expression profiling by high throughput sequencing Organism : Homo sapiens. The goal of this study is to determine the host response of human epithelial cells during infection with Francisella Tularensis.
PubMed News (NIH) 08/11/2020
Contributor : Shouhong Guang Series Type : Expression profiling by high throughput sequencing ; Non-coding RNA profiling by high throughput sequencing Organism : Caenorhabditis elegans. Background: Modification of RNAs, particularly at the terminals, is critical for various essential cell processes; for example, uridylation is implicated in tumorigenesis, proliferation, stem cell maintenance, and immune defense against viruses and retrotransposons.
PubMed News (NIH) 08/11/2020
Contributors : Peter Tsvetkov ; Timothy J Eisen ; Sven Heinrich ; Zarina Brune ; Erinc Hallacli ; Greg A Newby ; Can Kayatekin ; David Pincus ; Susan Lindquist Series Type : Expression profiling by high throughput sequencing ; Other Organism : Saccharomyces cerevisiae. The heat shock protein 90 (Hsp90) chaperone functions as a protein-folding buffer and plays a unique role promoting the evolution of new heritable traits.
PubMed News (NIH) 08/11/2020
Contributors : Peng Zhou ; Becky K Chan ; Yuk K Wan ; Chaya T Yuen ; Alan S Wong Series Type : Expression profiling by high throughput sequencing ; Other Organism : Homo sapiens ; synthetic construct. We present a CRISPR-based multi-gene knockout screening system and new toolkits for extensible assembly of barcoded high-order combinatorial guide RNA libraries en masse.
PubMed News (NIH) 08/11/2020
Contributors : Kitchener D Wilson ; Mohammed Ameen ; Lei Tian ; Yue Zhang ; Maxwell Mumbach ; Hongchao Guo ; Joseph C Wu Series Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencing ; Other Organism : Gorilla gorilla ; Homo sapiens ; Macaca mulatta ; Mus musculus ; Pan troglodytes. The protein-coding gene networks regulating heart development are well known.

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