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PubMed News (NIH) 08/15/2020
Contributors : Eva L Koch ; Frédéric Guillaume Series Type : Expression profiling by high throughput sequencing Organism : Tribolium castaneum. We studied plastic and evolutionary responses in gene expression of Tribolium castaneum after beetles’ exposure to new environments that differed from ancestral control conditions in temperature, humidity or both.
PubMed News (NIH) 08/15/2020
Contributors : S Makita ; T Hiroaki ; A Iwata ; H Nakajima Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. T-bet and STAT6 are critical factors for helper T cell differentiation in humans and mice. Additionally, polymorphisms in TBX21 (T-bet) and STAT6 are associated with the susceptibility of allergic diseases. However, precise mechanisms of the reciprocal regulation between T-bet and STAT6 in allergy remain unclear. To determine the reciprocal regulation in vivo, we investigated the phenotype of T-bet/STAT6 double-deficient (T-bet-/- STAT6-/-) mice. Unexpectedly, T-bet-/- STAT6-/- mice but not T-bet-/- mice or STAT6-/- mice spontaneously developed severe dermatitis. Not only eosinophils and mast ce.
PubMed News (NIH) 08/15/2020
Contributors : Yi Zhang ; Shengyi Liu Series Type : Expression profiling by high throughput sequencing Organism : Arabidopsis thaliana. PAB5 is a poly(A)-binding gene expressed in plant flowers, and PAB5 loss of function causes male sterility. We investigated the role of PAB5 in regulating poly(A) lengths and 5' cap efficiency in the context of pollen development.
PubMed News (NIH) 08/15/2020
Contributors : Isaac J Strong ; Xiaoyun Lei ; Fang Chen ; Kai Yuan ; Patrick H O’Farrell Series Type : Expression profiling by high throughput sequencing Organism : Drosophila melanogaster. Externally deposited eggs begin development with an immense cytoplasm and a single overwhelmed nucleus.
PubMed News (NIH) 08/15/2020
Contributors : Jolien Souffriau ; Steven Timmermans ; Tineke Vanderhaeghen ; Charlotte Wallaeys ; Kelly Van Looveren ; Lindsy Aelbrecht ; Sylviane Dewaele ; Jolien Vandewalle ; Evy Goossens ; Serge Verbanck ; Filip Boyen ; Melanie Eggermont ; Lindsey De Commer ; Riet De Rycke ; Michiel De Bruyne ; Raul Tito ; Marlies Ballegeer ; Sofie Vandevyver ; Tiago Velho ; Luis F Moita ; Tino Hochepied ; Karolien De Bosscher ; Jeroen Raes ; Filip Van Immerseel ; Rudi Beyaert ; Claude Libert Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. The cytokine TNF drives inflammatory diseases, e.g.
PubMed News (NIH) 08/15/2020
Series Type : Expression profiling by high throughput sequencing Organism : Homo sapiens. Adult stem cell identity, plasticity, and homeostasis are precisely orchestrated by lineage-restricted epigenetic and transcriptional regulatory networks. Here, by integrating super-enhancer and chromatin accessibility landscapes, we delineate core transcription regulatory circuitries (CRCs) of limbal stem/progenitor cells (LSCs) and find that RUNX1 and SMAD3 are required for maintenance of the corneal epithelial identity and homeostasis. RUNX1 or SMAD3 depletion inhibits PAX6 and induces LSCs to differentiate into epidermal-like type. RUNX1, PAX6 and SMAD3 (RPS) interact with each other and synergistically establish a CRC to govern the lineage-specific.
PubMed News (NIH) 08/15/2020
Contributors : Shukai Yuan ; Li Zhao Series Type : Expression profiling by high throughput sequencing Organism : Homo sapiens. Purpose: Next-generation sequencing (NGS) has revolutionized system-based cell pathway analysis. The purposes of this study were to compare and analyze the expression differences of skin squamous cell line A431 in stem cells after Mir-22 deletion. Methods: mRNA profiles of control and case cells were generated by deep sequencing, in duplicate, using Illumina NovaSeq 6000. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods:Bowtie2 to map clean reads to reference gene and use HISAT to reference genome.Bowtie2 parameters forSE reads:-q--phred64--sensitive--dpad0.
PubMed News (NIH) 08/15/2020
Contributors : Paul Basil ; Nancy Weigel Series Type : Expression profiling by high throughput sequencing Organism : Homo sapiens. CRPC remains AR dependent. There are multiple mechanisms for reactivation of AR including expression of constitutively active AR splices variant AR-V7 (AR3). Earlier studies suggest that though the variants regulate many of the same genes as AR, they also have unique targets. Another argument is that the variant is a “weak” AR. We have used an LNCaP cell line that expresses AR-V7 in response to doxycycline (LNCaP AR-V7) to compare the activities of the two isoforms and to identify differential regulation of target genes. We also used VCaP cell line that expresses AR-V7 in response to doxycycline (VCaP AR-V7) to v.
PubMed News (NIH) 08/15/2020
Contributors : Michał Kunc ; Marta Popęda ; Anna Szałkowska ; Magdalena Niemira ; Michał Bieńkowski ; Rafał Pęksa ; Aleksandra Łacko ; Barbara S Radecka ; Marcin Braun ; Joanna Pikiel ; Maria Litwiniuk ; Katarzyna Pogoda ; Ewa Iżycka-Świeszewska ; Adam Krętowski ; Anna J Żaczek ; Wojciech Biernat ; Elżbieta Senkus-Konefka Series Type : Non-coding RNA profiling by array Organism : Homo sapiens. In the current pilot study we aimed to identify differentially expressed miRNAs in two types of single hormone receptor-positive breast cancer [ER(+)/PgR(-) and ER(-)/PgR(+)] with further distinction into HER2-overexpressing/amplified and HER2-negative tumors in a well-established cohort collected at Medical University of Gdańsk.
PubMed News (NIH) 08/15/2020
Contributors : Barbara Mair ; Jelena Tomic ; Sanna N Masud ; Peter Tonge ; Alexander Weiss ; Matej Usaj ; Amy H Tong ; Jamie J Kwan ; Kevin R Brown ; Emily Titus ; Michael Atkins ; Katherin S Chan ; Lise Munsie ; Andrea Habsid ; Hong Han ; Marion Kennedy ; Brenda Cohen ; Gordon Keller ; Jason Moffat Series Type : Other Organism : Homo sapiens. Human pluripotent stem cells (hPSCs) provide an invaluable tool for modeling diseases and hold promise for regenerative medicine.
PubMed News (NIH) 08/15/2020
Contributors : Etienne Bucher ; Kateryna Fal ; Emilija Histova Series Type : Expression profiling by high throughput sequencing Organism : Arabidopsis thaliana. Chromatin and its covalent modifications play important roles during development. However, how different types of chromatin regulators individually control gene expression in different cell types has so far not been investigated in detail. To explore their role in these processes we performed forward and reverse genetic experiments using an epigenetically silenced reporter gene in Arabidopsis. We found three chromatin regulators, each involved in different epigenetic pathways, to control reporter gene expression in distinct tissues and cell-types. Here we report on the roles of the R.

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