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PubMed News (NIH) 12/15/2019 23:00
Contributors : Erika Andrade ; Johana Alvarez Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. TRAP translational profiling is a method that allows investigators to genetically characterize specific cell types in complex tissues such as mouse brain.
PubMed News (NIH) 12/15/2019 23:00
Contributors : Eicke Latz ; Mario Lauterbach ; Christian Kolbe ; Timo Hess ; Jan Gehlen Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. TLR activation induces inflammatory responses in macrophages by activating temporally defined transcriptional cascades within the first hours after stimulation.
PubMed News (NIH) 12/15/2019 23:00
Contributors : Catherine J Wu ; Derin B Keskin Series Type : Expression profiling by high throughput sequencing Organism : Homo sapiens. Prediction of HLA epitopes is important for the development of cancer immunotherapies and vaccines. However, current prediction algorithms have limited predictive power, in part because they were not trained on high-quality epitope datasets covering a broad range of HLA alleles. To enable prediction of endogenous HLA class I-associated peptides across a large fraction of the human population, we used mass spectrometry to profile >185,000 peptides eluted from 95 HLA-A, -B, -C and -G mono-allelic cell lines. We identified canonical peptide motifs per HLA allele, unique and shared binding submotifs across alle.
PubMed News (NIH) 12/15/2019 23:00
Contributors : Erika Andrade ; Johana Alvarez Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. TRAP translational profiling is a method that allows investigators to genetically characterize specific cell types in complex tissues such as mouse brain.
PubMed News (NIH) 12/15/2019 23:00
Contributors : Erika Andrade ; Johana Alvarez Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. Using translational profiling we obtained RNA-Seq data from total RNA present in the dorsal striatum and accumbens nucleus of older Drd1-EGFP/Rpl10a (CP73) mice.
PubMed News (NIH) 12/15/2019 23:00
Contributor : Aaron Zorn Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. We dissected foreguts from Gli2-/-,Gli3-/- and Gli2+/-,Gli3+/- mouse embryos at embryonic day 9.5. Three foreguts were obtained for each group and each foregut was individually processed for bulk RNA extraction and library preparation. Sequencing results identified Gli positively and negatively regulated genes in the foreguts.
PubMed News (NIH) 12/15/2019 23:00
Contributors : Andreas Gorlich ; Ines Ibanez-Tallon Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. TRAP translational profiling is a method that allows investigators to genetically characterize specific cell types in complex tissues such as mouse brain.
PubMed News (NIH) 12/15/2019 23:00
Contributors : Xipeng Wang ; Xiang Ying Series Type : Non-coding RNA profiling by array Organism : Homo sapiens. reports shows that EOC cell under hypoxia condition shows high malignancy. So we use the Arraystar Human LncRNA Microarray V3.0 to verify the difference expression of LncRNA in the exosomes drived from skov-3 cells which cultured in nomor/hypoxia condition.
PubMed News (NIH) 12/15/2019 23:00
Contributors : Philipp F Popp ; Thorsten Mascher Series Type : Expression profiling by high throughput sequencing Organism : Bacillus subtilis subsp. subtilis str. 168. To obtain global cellular response of Bacillus subtilis WT W168 against the intrinsically produced antimicrobial peptide YydF, we performed RNA-seq experiments. For this, we synthesized YydF, extrinsically added 0.5µM to logarithmic phase growing cells and harvested cells after 10 min exposure. Experiments were performed in triplicates and non-induced Bacillus subtilis WT W168 was used as reference condition.
PubMed News (NIH) 12/15/2019 23:00
Contributors : Erika Andrade ; Johana Alvarez Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. TRAP translational profiling is a method that allows investigators to genetically characterize specific cell types in complex tissues such as mouse brain.
PubMed News (NIH) 12/15/2019 23:00
Contributors : Erika Andrade ; Johana Alvarez Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. TRAP translational profiling is a method that allows investigators to genetically characterize specific cell types in complex tissues such as mouse brain.

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