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PubMed News (NIH) 10/23/2020
Contributors : Marta San Martin-Alonso ; Maria E Soler-Oliva ; Maria Garcia-Rubio ; Tatiana Garcia-Muse ; Andres Aguilera Series Type : Genome binding/occupancy profiling by high throughput sequencing ; Other Organism : Saccharomyces cerevisiae. R loops induce transcription-replication (T-R) conflicts, but recent reports have suggested that R-loops are caused by T-R conflicts.
PubMed News (NIH) 10/23/2020
Contributors : Mark Ziemann ; Gavin C Higgins ; Sih M Tan ; Runa S Lindblom ; Cesare Granata ; Matthew Snelson ; Vicki Thallas-Bonke ; Assam El-Osta ; Carlos D Baera-Garza ; Stuart T Caldwell ; Richard C Hartley ; Thomas Krieg ; Mark E Cooper ; Michael P Murphy ; Melinda T Coughlan Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. Diabetic kidney disease (DKD) remains the number one cause of end-stage renal disease in the western world.
PubMed News (NIH) 10/23/2020
Contributors : Natalie R Powers ; Tanmoy Bhattacharyya Series Type : Genome binding/occupancy profiling by high throughput sequencing ; Third-party reanalysis ; Other Organism : Mus musculus. The locations of mammalian recombination hotspots are determined by PRDM9, a zinc finger histone methyltransferase that locally trimethylates histone H3 at residues K4 and K36.
PubMed News (NIH) 10/23/2020
Contributors : Russell T Hepple ; Vijayendran Chandran Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. Purpose: The goal of this study was to analyze the gene expression levels by RNA sequencing from the plantaris muscle of mice exposed to normal air or tobacco smoke for 16 weeks.
PubMed News (NIH) 10/23/2020
Contributors : Joanna Kim ; John Cooper Series Type : Expression profiling by high throughput sequencing Organism : Homo sapiens. Arrangements of juntional proteins of endothelial monolayers were altered by both septin 2 KD and TNF-a treatment. Therefore, differential gene expression profiles by septin 2 KD comapred to control and TNF-a treatment compared to untreat were analyzed using RNA-seq.
PubMed News (NIH) 10/23/2020
Contributors : Miyu Kamimura ; Azusa Sasaki ; Shimpei Watanabe ; Shiho Tanaka ; Akiko Fukukawa ; Kazuya Takeda ; Yasushi Nakamura ; Takako Nakamura ; Kouji Kuramochi ; Yui Otani ; Fumio Hashimoto ; Kanji Ishimaru ; Tomoaki Matsuo ; Shigehisa Okamoto Series Type : Expression profiling by array Organism : Homo sapiens. Methylthioacetic acid (MTA) is thought to potentially have an anticancer activity because it promotes the differentiation of unorganized cell mass into dome tissues in RCM-1 human colorectal cancer cell line.
PubMed News (NIH) 10/23/2020
Contributor : Weibo Yu Series Type : Expression profiling by array Organism : Homo sapiens. HUC model includes three cell lines: HUC-BC, HUC-PC, and MCT11. They were all originally derived from human uroepithelium. However, three cell lines have different malignant potential. We here performed micro array to examine the differences in gene expression among three cell lines.
PubMed News (NIH) 10/23/2020
Contributors : Daniel P Krummel ; Maxwell Xu ; Mario Medvedovic ; Soma Sengupta Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. Most metastatic melanoma patients show variable responses to radiotherapy and do not benefit from immune checkpoint inhibitors (ICIs).
PubMed News (NIH) 10/23/2020
Contributors : Weilin Chen ; Yue Xiao Series Type : Expression profiling by array Organism : Homo sapiens. Glioma is characterized by high migration and invasion, and the relative molecular mechanism is still poor. Accumulating studies demonstrated that ubiquitin specific protease 39 (USP39) played an oncogenic role in several cancers. Here, we investigate USP39 expression and function in human glioma. Oncomine database analysis revealed high USP39 expression in glioma and elevated USP39 expression correlated significantly with poor overall survival. Knockdown of USP39 significantly inhibited the migration and invasion of U251 and U87 cells. The gene expression profile was executed to screen the target molecules of USP39. The result showed t.
PubMed News (NIH) 10/23/2020
Contributors : Jeremy P Blaydes ; Matthew J Rose-Zerilli Series Type : Expression profiling by high throughput sequencing Organism : Homo sapiens. We report the single-cell RNA sequencing data obtained from MDA-MB-231 breast cancer cells cultured in standard DMEM (high glucose) media, or adapted to culture in standard DMEM (high glucose) media containing 2 mM metformin, and then cultured as mammospheres.
PubMed News (NIH) 10/23/2020
Contributor : Hans I Harn Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. Tissue regeneration is a process that recapitulates the molecular and mechanical aspects of development and evolution. We use the wound-induced hair neogenesis (WIHN) model to investigate the mechanical and molecular responses of the laboratory (Mus) and African spiny (Acomys) mice. Laboratory and spiny mice showed an opposite trend of spatiotemporal morphogenetic field for WIHN during wound healing, and wound stiffness gradient across the whole wound bed predicated pattern of hair formation. Using bulk and single-cell RNA-seq analysis and K14-Cre-Twist1 transgenic mice, we identified the central role of the Twist1 pathway as t.
PubMed News (NIH) 10/23/2020
Contributors : Debosree Pal ; Neha C Venkata ; Utsa Bhaduri ; C Subbulakshmi ; Satyanarayana M Rao Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. Earlier studies from our lab have characterized lncRNA Mrhl extensively in the context of male germ cell meiotic commitment and spermatogenesis, unraveling a negative feedback loop between Mrhl and a highly conserved context-dependant developmental pathway such as the WNT pathway.

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