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PubMed News (NIH) 10/23/2019
Contributors : Malcolm Eaton ; Matthew Workentine Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. We used RNA sequencing to identify differentially expressed genes in whole mouse placentas. Pregnant mice were fed either a 20% or 6% protein (isocaloric) two-weeks before mating and throughout pregnancy. Placental lysates are derived from C57BL/6 conceptuses at E16.5 of gestation.
PubMed News (NIH) 10/23/2019
Contributors : Zlata Gvozdenov ; Brian C Freeman Series Type : Genome binding/occupancy profiling by high throughput sequencing Organism : Saccharomyces cerevisiae. We report the application of DHS-Seq and digital genomic footprinting to study chromatin changes and transcription factor-DNA binding upon long-term Hsp90 depletion utilizing the temperature-sensitive allele G170D.
PubMed News (NIH) 10/23/2019
Contributors : S Cowman ; V See Series Type : Expression profiling by high throughput sequencing Organism : Homo sapiens. Hypoxia is known to impact DNA repair mechanisms. The primary aim of this study was to examine the impact of chronic (5 days) hypoxia on the expression of DNA repair genes in glioblastoma and medulloblastoma cell lines. The NanoString nCounter Vantage™ RNA Panel for DNA Damage and Repair (LBL-10250-03) was utilised to analyse gene expression using total RNA extracted from cells.
PubMed News (NIH) 10/23/2019
Contributors : Junbo Liang ; Hong Zhao ; Bill H Diplas ; Song Liu ; Jianmei Liu ; Dingding Wang ; Yan Lu ; Qing Zhu ; Jiayu Wu ; Wenjia Wang ; Hai Yan ; Yi-Xin Zeng ; Xiaoyue Wang ; Yuchen Jiao Series Type : Other Organism : Homo sapiens. we performed a genome-wide synthetic lethal screen, using CRISPR-Cas9 genome editing, to identify potential therapeutic targets specific for ATRX-mutated cancers.
PubMed News (NIH) 10/23/2019
Contributors : Jordan Metz ; Nicholas Hornstein ; Sohani D Sharma ; Jeremy Worley ; Peter Sims Series Type : Expression profiling by high throughput sequencing Organism : Homo sapiens. Protein synthesis is dysregulated in many diseases, but we lack a systems-level picture of how signaling molecules and RNA binding proteins interact with the translational machinery, largely due to technological limitations.
PubMed News (NIH) 10/23/2019
Contributors : Ramil Hakim ; Ruxandra Covacu ; Arvid Frostell ; Sreenivasa R Sankavaram ; Mikael Svensson ; Lou Brundin Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. We evaluated the importance of histocompatibility of transplanted MSCs in terms of therapeutic potential.
PubMed News (NIH) 10/23/2019
Contributors : Robert Denver ; Christopher Sifuentes ; Yasuhiro Kyono ; Samhitha Raj ; Nicolas Buisine ; Laurent Sachs Series Type : Methylation profiling by high throughput sequencing Organism : Xenopus tropicalis. Changes in DNA methylation in the preoptic area/hypothalamus/thalamus of Xenopus tropicalis tadpoles were analyzed using Methylated DNA Capture sequencing (MethlCap-seq) at four stages of metamorphosis.
PubMed News (NIH) 10/23/2019
Contributors : Kuniko Nakajima ; Michio Ono ; Uroš Radović ; Selma Dizdarević ; Shin-ichi Tomizawa ; Kazushige Kuroha ; Go Nagamatsu ; Ikue Hoshi ; Risa Matsunaga ; Takayuki Shirakawa ; Takeyuki Kurosawa ; Yasunari Miyazaki ; Masahide Seki ; Yutaka Suzuki ; Haruhiko Koseki ; Masataka Nakamura ; Toshio Suda ; Kazuyuki Ohbo Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. Respiratory failure is a life-threatening problem for pre-term and term infants, yet many causes remain unknown.
PubMed News (NIH) 10/23/2019
Contributors : Dagmara Korona ; Bettina Fischer ; Steve Russell Series Type : Expression profiling by high throughput sequencing Organism : Drosophila melanogaster. Purpose: The goals of this study are to compare the embryonic transcriptome of embryos lacking major shaggy transcript isoforms to determine the impact of particular proteoforms on gene expression and identify affected pathways.
PubMed News (NIH) 10/22/2019
Contributors : Hideyuki Takahashi ; Judith A Varner Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. We characterized myeloid cell populations in murine lung cancer models by single-cell sequencing. These data were in line with our previous findings about myeloid cell subsets in tumor microenvironment.
PubMed News (NIH) 10/22/2019
Contributors : Guoqiang Wan ; Sihao Gong Series Type : Expression profiling by high throughput sequencing Organism : Mus musculus. We report application of RNA-seq for transcriptomic analyses of cochlear tissues from wildtype and DFNA15 deafness mouse model Pou4f3(Δ/+) to reveal gene expressions altered by Pou4f3 mutation.

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